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PCR Primer Design for Cloning of the Porcine Reproductive and Respiratory Syndrome Virus Gene

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01:58 min

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May 17th, 2024

DOI :

10.3791/201619-v

May 17th, 2024

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Ming Yang*¹^,², Lijuan Cui*³, Xuesha Liu¹

¹College of Life Sciences, China West Normal University, ²Sichuan Sports College, ³Pathology Department, Suining Central Hospital

* These authors contributed equally

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PCR Primer Design

PRRSV Gene Sequence

Fragment Junctions

Specific Sequence Primers

Restriction Site

Vector Overhang Sequences

Agarose Gel Electrophoresis

XDNA Loading Buffer

Gene Amplification

来自系列

Cloning the Full-Length PRRSV Gene in the pVAX1 Expression Vector

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