JoVE Logo

登录

需要订阅 JoVE 才能查看此.

Generating Neurons and Glial Cells from Human Induced Pluripotent Stem Cell-Derived Embryoid Bodies

-- views • 1:13 min

成績單

Take embryoid bodies, or EBs, derived from human induced pluripotent stem cells. These EBs are composed of three germ layers.

Transfer the EBs to a matrix-coated culture dish containing a suitable medium and incubate.

The matrix enables the EBs to attach to the culture dish.

The growth factors and nutrients in the medium facilitate the ectodermal cells of the EBs to form neuroepithelial aggregates arranged in a circular pattern termed a rosette.

Cut the rosette fragments and detach them from the dish.

Collect the fragments and spin them down.

Discard the supernatant and resuspend the fragments.

Using a pipette, move the fragments up and down to dissociate them.

Add the desired number of cells to a matrix-coated well plate containing a differentiation medium.

The components of the differentiation medium facilitate the neuroepithelial cells to transform into fully functional neuronal and glial cells, the primary cells of the nervous system.

article

02:47

Generating Neurons and Glial Cells from Human Induced Pluripotent Stem Cell-Derived Embryoid Bodies

相关视频

16 Views

JoVE Logo

政策

使用条款

隐私

科研

教育

关于 JoVE

版权所属 © 2025 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。