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JoVE Journal

Immunology and Infection

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Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

56.5K Views

08:01 min

May 2nd, 2011

May 2nd, 2011

56,500 Views

0:05

Title

1:32

Intratracheal Injection

3:11

Harvesting and Processing Lung Draining Lymph Nodes and Lungs for Flow Cytometry

6:33

Representative Flow Cytometric Analyses of Lung Draining Lymph Nodes After Intratracheal Injection

7:39

Conclusion

We illustrate non-surgical delivery of test materials into the lungs of anesthetized mice via the trachea. This method permits lung exposure to bacterial and viral pathogens, cytokines, antibodies, beads, chemicals, or dyes. We further describe harvesting and processing of lungs and lung draining lymph nodes (LDLNs) for flow cytometry.

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