Collection of Colonic Tissue Samples and Assessment of Colitis Severity
3:58
Sample Preparation for Myeloperoxidase (MPO) Assay
5:09
Quantification of Inflammatory Markers
6:01
Evaluating the Severity of Acute Colitis in DSS-treated Animals
8:23
Conclusion
Experimental models of inflammatory bowel disease have allowed us to examine the complex innate and adaptive immune responses associated with pathogenesis. Using histological scoring, quantification of pro-inflammatory cytokines and myeloperoxidase activity, one can begin to assess these responses seen in inflammatory bowel disease.
