Setting up the Extracellular Glass Electrodes for Soma Recordings
8:35
Setting up the Suction Electrodes for the Nerve Recordings
9:41
Setting up the Force Transducer to Measure the I1/I3 Muscle Contraction
10:38
Results: Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica
13:19
Conclusion
In animals with large identified neurons (e.g. mollusks), analysis of motor pools is done using intracellular techniques1,2,3,4. Recently, we developed a technique to extracellularly stimulate and record individual neurons in Aplysia californica5. We now describe a protocol for using this technique to uniquely identify and characterize motor neurons within a motor pool.
