Site-specific Genetic Incorporation of Unnatural Amino Acids into GPCRs
3:15
Mapping a Ligand Binding Site Using Targeted Photocrosslinking
7:12
Targeted Epitope Tagging and Cell Surface Enzyme-linked Immunosorbent Assay (ELISA)
10:23
Bioorthogonal Fluorescent Labeling of a GPCR
12:10
Results: Versatile Applications of Site-specific azF Incorporation into GPCRs
We genetically-encode the unnatural amino acid, p-azido-L-phenylalanine at various targeted positions in GPCRs and show the versatility of the azido group in different applications. These include a targeted photocrosslinking technology to identify residues in the ligand-binding pocket of a GPCR, and site-specific bioorthogonal modification of GPCRs with a peptide-epitope tag or fluorescent probe.
