Designing a Peptide Array and Blocking Non-specific Binding
3:04
Incubating the Array with the Protein and Antibody
5:09
Reading the Array
5:57
Results: Screening a Peptide Array for Binding of STIL Intrinsically Disordered Region to CHFR Protein
7:18
Conclusion
Peptide array screening is a high throughput assay for identifying protein-protein interaction sites. This allows mapping multiple interactions of a target protein and can serve as a method for identifying sites for inhibitors that target a protein. Here we describe a protocol for screening and analyzing peptide arrays.
