Evaluation of Bacterial Concentration and Single Primer Colony PCR
5:34
Eukaryotic Cell Challenge with Coxiella Mutants and Monitoring of Intracellular Growth
7:17
Preparation of Samples for Automated Image Acquisition
9:25
Results: Viability and Infectivity of Coxiella Transposon Mutants
11:14
Conclusion
Coxiella burnetii is an obligate intracellular Gram-negative bacterium responsible for the zoonotic disease Q fever. Here we describe methods for the generation of Coxiella fluorescent transposon mutants as well as the automated identification and analysis of the resulting internalization, replication and cytotoxic phenotypes.
