Cytotoxic Neutrophil Isolation from the Blood of Tumor-bearing Mice
3:43
Circulating Neutrophil Isolation from Cancer Patients
5:07
Thioglycollate- or Zymosan-elicited Neutrophil Isolation
6:08
Cytological Staining of Isolated Neutrophils
6:48
In vitro Luciferase Analysis of Isolated Neutrophil Anti-tumor Activity
8:15
Results: Representative Neutrophil Functionality
9:49
Conclusion
Neutrophils play an important role not only in host defense against invading microorganisms, but are also involved in the immune surveillance of tumor cells. Here, we describe techniques related to the isolation of neutrophils with anti-tumor properties and methods for monitoring anti-tumor neutrophil function in vitro and in vivo.
