Data Mining for Transcription Factor Binding Sites in the Promoter Regions of MicroRNA
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Producing a Lentivirus-containing shRNA in 293 Human Embryonic Kidney Cell Line
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Transfection of a Cell with Lentivirus-containing shRNA
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Results: shRNA Down-regulation of STAT3 MicroRNA Leads to Significant Expression Changes in CLL Cells
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Conclusion
副本
The overall goal of this methodology is to describe how a transcription factor of interest regulates the microRNA transcriptome. This method can help answer key questions on whether transcription factors that modulate transcription of protein codi
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Herein we propose a strategy to study the effect of a transcription factor of interest on the microRNA transcriptome using publically available data, computational resources and high throughput data from microRNA arrays after transfecting cells with small hairpin (sh)RNA targeting a transcription factor of interest.
