Prepare Medium, Seed Cells in 48-well Plates, and Carry Out Virus-cell Attachment
2:52
Viral RNA Extraction
4:22
qRT-PCR Measurement of Viral Genome
6:38
Results: Arenavirus Cell Attachment Measurements
8:08
Conclusion
副本
The overall goal of this protocol is to quantitatively measure arenavirus particle attachment to host cells. The main advantage to this technique is its high sensitivity. It can also be adapted to measure virion endocytosis, as well as the release
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The first step in the arenavirus life cycle is attachment of viral particles to host cells. We report a quantitative (q)RT-PCR-based assay for ultrasensitive detection and quantitation of arenavirus attachment events.
