Preparation of Injection Solutions and Microinjection of Embryos
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Fixation and Immunostaining of Embryos
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Results: Examples of Embryos Targeted at the 8-cell Stage
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Conclusion
副本
The overall goal of this procedure is to target microinjections to the developing xenopus kidney. This method can help address key questions in the field of developmental biology such as how the kidney forms and what genes are important for this p
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Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.