Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

0:39

Generation of Retroviral Stock

2:20

Viral Infection of HEK293T Cells

4:30

Genomic DNA Fragmentation

5:33

Ligation of Linker Oligonucleotide to Fragmented Genomic DNA

6:58

Amplification of Viral LTR-host Genomic DNA Junctions by Semi-nested PCR

8:02

Results: Sequence Logos Depicting HIV-1 Base Preferences from Representative Experiment Libraries

8:52

Conclusion