Subcutaneous (SQ) and Visceral (VIS) Fat Pad Isolation
2:35
Fat Pad Collagenase Digestion
4:20
Magnetic Cell Separation
6:31
Results: Fat Depot-dependent Expression of Collagenolytic Matrix Metalloproteinases (MMPs)
7:52
Conclusion
副本
The overall goal of this procedure is to isolate Sca1 positive mesenchymal stem cells from various mouse fat pads. The main advantage of this technique is that by using the immunomagnetic beads-based cell sorting adipose-derived stem cells from di
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We present the techniques required to isolate the stromal vascular fraction (SVF) from mouse inguinal (subcutaneous) and perigonadal (visceral) adipose tissue depots to assess their gene expression and collagenolytic activity. This method includes the enrichment of Sca1high adipose-derived stem cells (ASCs) using immunomagnetic cell separation.