The overall goal of these procedures is to visualize and track the progression of somatic cell reprogramming into induced pluripotent stem cells or iPSC. This method can be used to answer key questions in the iPSC field, such as how reprogramming
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The protocol presented in this study describes methods for the real-time monitoring of reprogramming progression via the kinetic measurement of positive and negative pluripotent stem cell markers using flow cytometry analysis. The protocol also includes the imaging-based assessment of morphology, and marker or reporter expression during iPSC generation.
