The overall goal of this procedure is to perform long-term microscopic imaging and tracking of neutrophils in vascular structures within the murine bone marrow through an implanted chronic window chamber over the femoral bone. This method can help
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The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.
