Results: Quantification and Imaging of Hemocytes, and Imaging of Dissected and Immunostained Lymph Gland Tissue
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Conclusion
副本
The overall goal of these procedures, is to analyze various components of the Drosophila hematopoietic system with an emphasis on lymph gland dissection and mounting for immunohistochemistry. These methods can help answer key questions in the Dros
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Drosophila and mammalian hematopoietic systems share many common features, making Drosophila an attractive genetic model to study hematopoiesis. Here we demonstrate dissection and mounting of the major larval hematopoietic organ for immunohistochemistry. We also describe methods to assay various larval hematopoietic compartments including circulating hemocytes and sessile crystal cells.
