The overall goal of this method is the quantify transcript accumulation in specific cells or cellular domains so that comparisons can be made between different domains or between equivalent domains in mutant and wild type samples. This method can
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Many developmentally important genes have cell- or tissue-specific expression patterns. This paper describes LM RNA-seq experiments to identify genes that are differentially expressed at the maize leaf blade-sheath boundary and in lg1-R mutants compared to wild-type. The experimental considerations discussed here apply to transcriptomic analyses of other developmental phenomena.
