Results: Rapid Differentiation for Visualization and Analysis
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Conclusion
副本
The overall goal of this protocol is to differentiate human pluripotent stem cells into human trophoblastic cells using bone morphogenic 4 and inhibitors of the active and nodal signaling pathways to study the early events of human placental forma
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Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.
