Preparation of Paper Layers and Nylon Membrane Layer
2:51
Creating Hydrophobic Barriers in the Printed Layer
3:53
Preparation of Adhesive Layers and Bqacking of Device Layers with Adhesive
4:52
Treatment of Conjugate Layer with Reagents for Immunoassays Prior to Device Assembly
5:38
Treatment of Lateral Channel and Capture Layer with Reagents for Immunoassays Prior to Device Assembly
6:52
Assembly of Three-dimensional Paper-based Microfluidic Devices
8:33
Performing a Paper-based Immunoassay
9:17
Results: Fabrication of Paper-based Immunoassays
10:33
Conclusion
Transcript
The overall goal of this protocol is to demonstrate our process for fabricating three-dimensional paper-based devices, which we use as a platform to develop point of care immunoassays. This method provides a paper-based microfluidics platform with
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We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.
