Imaging Stained Neurons within Thick Brain Sections
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Results: Photomicrographs of Stained Neurons in the Anterior Cingluate Area 1
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Conclusion
副本
The overall goal of this procedure is to determine the effective treatment on the morphology of neurons within the rodent brain. This method can help answer key questions in the neuroscience field, such as the normal and experimentally manipulated
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We present a protocol for using the Golgi-Cox staining method in thick brain sections, in order to visualize neurons with long dendritic trees contained within single tissue samples. Two variants of this protocol are also presented that involve cresyl violet counterstaining, and the freezing of unprocessed brains for long-term storage.
