Results: Representative En Face Immunofluorescent Imaging
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Conclusion
副本
The overall goal of this procedure is to prepare the mouse aorta and carotid artery for en face immuno staining after left carotid artery ligation. This method allows us to study organization, each protein expression, and possible state of antral
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A procedure for making en face preparations of the mouse carotid artery and aorta is described. Such preparations, when immunofluorescently stained with specific antibodies, enable us to study localization of proteins and identification of cell types within the entire vascular wall by confocal microscopy.