The overall goal of this procedure is to measure basal and forskolin-stimulated lipolysis, using inguinal adipose tissue. This method can help answer key questions in field of adipose biology about lipolysis and triglyceride metabolism. The main a
Sign in or start your free trial to access this content
This protocol describes the method of determining basal and forskolin-stimulated lipolysis in inguinal fat pads obtained from normal chow diet (NCD) or high fat diet (HFD) ± capsaicin fed wild type mice. As an index for lipolysis, glycerol release was measured from inguinal adipose fat pads.
