Whitefly Rearing, Cryptic Species Identification, and Endosymbiont Identification and Localization
2:11
Whitefly Bacteriome Dissection and Purification
3:47
Amplification of Endosymbiont Genomes
5:02
Results: Extraction and Genome Sequencing of Endosymbionts from the Whitefly Bemisia tabaci
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Conclusion
副本
The overall goal of this experiment is to extract endosymbionts from tiny insects for further experiments. This method can help answer key questions in the entomology and the macrobiology view because most endosymbionts cannot be cultured in vitro
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Here, we present a protocol to isolate endosymbionts from the whitefly Bemisia tabaci through dissection and filtration. After amplification, the DNA samples are suitable for subsequent sequencing and study of the mutualism between endosymbionts and whitefly.
