Transfection of Cardiomyocytes with Fluorescently Labeled miRNA
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Applying 3D-FRAP Microscopy and Data Analysis
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Results: 3D FRAP Analysis of Gap Junction-dependent Transfer of miRNA
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Conclusion
副本
The overall goal of this procedure is to investigate the gap junction-dependent transfer of micro RNA in living cardiomyocytes. This method can help to address the role of micro RNA for the intercellular signal transaction system. The main advanta
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Here, we describe the application of three-dimensional fluorescence recovery after photobleaching (3D-FRAP) for the analysis of the gap junction-dependent shuttling of miRNA. In contrast to commonly applied methods, 3D-FRAP allows for the quantification of the intercellular transfer of small RNAs in real time, with high spatio-temporal resolution.
