Preparation of Fluorescent Nano-diamond-coated Coverslips
2:49
Activation of Jurkat T Cells
4:15
Imaging Activated Jurkat T Cells Using madSTORM
7:00
Results: Multiplexed Super-resolution Image of Proteins in an Activated T Cell
7:54
Conclusion
副本
The overall goal of this imaging protocol is to visualize multiple proteins in an activated T cell, using single molecule localisation microscopy. This method can help answer key questions in the T cell activation field such as, the single molecul
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We demonstrate a method to image multiple molecules within heterogeneous nano-structures at single molecule accuracy using sequential binding and elution of fluorescently labeled antibodies.
