Picking Fully Reprogrammed Colonies for Clonial Expansion
6:43
Clonal Expansion and Maturation of iPSCs
7:47
Results: Morphological Progression of Cells During Reprogramming
8:43
Conclusion
副本
The overall goal of this procedure is to efficiently derive induced pluripotent stem cells by means of episomal reprogramming from two different kinds of fetal stem cells. This method can help provide a source of pluripotent stem cells for tissue
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This protocol describes the reprogramming of primary amniotic fluid and membrane mesenchymal stem cells into induced pluripotent stem cells using a non-integrating episomal approach in fully chemically defined conditions. Procedures of extraction, culture, reprogramming, and characterization of the resulting induced pluripotent stem cells by stringent methods are detailed.