Generating Embryos Through Natural Mating and Staging Embryos
2:22
Whole Embryo Dissociation, RNA Extraction and Purify High-quality RNA
4:55
Sorting Differentially Expressed Genes
7:00
Determine Enriched Pathways
7:57
Generation of Pathway Networks and Determination of Enriched GO Terms
9:12
Results: RNAseq-based Zebrafish Genome Expression Data for Alstrom and Bardet Biedl Syndromes
10:47
Conclusion
副本
The overall goal of this RNASeq analysis in zebrafish larval samples is to identify gene expression profiles for zebrafish embryos and larvae and to make quantitative comparative statements about gene expression changes between samples. This metho
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This protocol presents an approach for whole transcriptome analysis from zebrafish embryos, larvae, or sorted cells. We include isolation of RNA, pathway analysis of RNASeq data, and qRT-PCR-based validation of gene expression changes.
