The overall goal of this retina preparation method is to use video microscopy to measure mouse retinal arterial reactivity. This method can help answer key questions in the field of retina blood flow regulation and may help further our understandi
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Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.
