Overlapping Peptide (OLP) Library Generation and T Cell Priming with OLP Pool-pulsed Dendritic Cells (DCs) Protocol
3:43
Re-stimulation of OLP Pool-primed CD8+ T Cells Using Macrophages Pulsed with the OLP Pool
6:19
Results: Identification of a 9-mer Qa-1 Epitope in Myelin Oligodendrocyte Glycoprotein (MOG)
7:32
Conclusion
副本
The overall goal of this procedure is to identify Qa-1 epitopes in proteins. This method can help answer key questions in the field of immune regulation and immunotherapy. The main advantages of this technique are that mapped peptides are known to
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Qa-1 (HLA-E in human) belongs to a group of non-classical major histocompatibility complex 1b molecules. Immunization with Qa-1-binding epitopes has been shown to augment tissue-specific immune regulation and ameliorate several autoimmune diseases. Herein we describe an overlapping peptide library strategy for the identification of Qa-1 epitopes in a protein.
