Acute Mechanical Stimulation Using an Orbital Shaker
4:18
Acute Mechanical Stimulation Using a Microfluidic Chamber
6:33
Applying Pharmacological Treatments Using the Microfluidic Chamber
7:48
Results: Observed Shear Force Responses in D. discoideum
9:18
Conclusion
副本
The overall goal of this procedure is to examine the activity of various components of the intracellular signal transduction network following brief mechanical perturbation. This method can help answer key questions in the field of cell migration
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Here we describe methods for assessing cellular response to acute mechanical stimulation. In the microscopy-based assay, we examine localization of fluorescently-labeled biosensors following brief stimulation with shear flow. We also test activation of various proteins of interest in response to acute mechanical stimulation biochemically.
