Preparation of Mouse Bone Marrow Derived Macrophages (BMDMs) and BSA-fatty Acid Conjugate
4:31
Saturated Fatty Acids Induce Cell Death of Mouse Bone Marrow Derived Macrophages
6:04
Intracellular Ceramide Staining
7:03
Results: Fatty Acid-induced Macrophage Cell Death and Ceramide Production
7:47
Conclusion
副本
The overall goal of this experiment, in which macrophages are treated with saturated fatty acids, is to demonstrate that saturated fatty acid-induced macrophages cell death is associated with accumulated cellular levels of Ceramides. This method c
Sign in or start your free trial to access this content
We illustrate a straight-forward method to derive murine primary macrophages from bone marrow cells and a simple method to prepare BSA-fatty acid conjugates. Then we demonstrate that saturated fatty acids can induce macrophage cell death, and such cell death is positively associated with cellular accumulation of ceramide levels.
