Cell Culture, Plasmid Transfection, and Chemical Induction
1:45
Quantification of Rapamycin-indiced 5-hydroxymethylcystosine (5hmc) Production by Immunostaining
3:08
Dot-blot Assay Quantify 5hmc and 5-methylcystosine (5mc) Amounts
7:03
Results: Validation of Chemically Induced 5hmc Levels
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Conclusion
副本
The overall goal of this series of experiments is to introduce an engineered split-TET2 enzyme called CiDER into mammalian cells for inducible DNA modification such as hydroxymethylation as well as epigenetic remodeling. This method can help answe
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Detailed protocols for introducing an engineered split-TET2 enzyme (CiDER) into mammalian cells for chemical inducible DNA hydroxymethylation and epigenetic remodeling are presented.