Differentiation of Human iPSCs to Hepatocyte-like Cells and Small Molecule Screening
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Results: Differentiation of iPSCs and Identification of Small Molecules for Drug Discovery
11:12
Conclusion
Transcript
The overall goal of this experiment is to differentiate human induced pluripotent stem cells into hepatocytes-like cells in a manner that is suitable for high-throughput screening. Liver cells are difficult to grow in the laboratory, however they
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The protocol presented here describes a platform for identifying small molecules for the treatment of liver disease. A step-by-step description is presented detailing how to differentiate iPSCs into cells with hepatocyte characteristics in 96-well plates, and to use the cells to screen for small molecules with potential therapeutic activity.
