Inserting Sequences of Interest into BIBAC-GW Binary Vector
2:37
Arabidopsis Transformation
5:37
Characterizing Transgenics for the Number and Integrity of T-DNA Integrations
8:19
Results: Analysis of the Number of T-DNA Insertions in Plants Transformed with BIBAC-Bar-GW Carrying a Reporter Sequence
10:01
Conclusion
副本
The overall goal of this methodology is to generate transgenic plants carrying intact, single-copy insertions of a transfer DNA, or T-DNA, in an efficient manner. The BIBAC-Gateway vector is a valuable tool for generating transgenic plants that ca
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Using a pBIBAC-GW binary vector makes generating transgenic plants with intact single-copy insertions, an easy process. Here, a series of protocols is presented that guide the reader through the process of generating transgenic Arabidopsis plants, and testing the plants for intactness and copy number of the inserts.
