Results: Analysis of Protein Recruitment to DNA Lesions Using 405 nm Laser Micro-irradiation
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Conclusion
Transcript
The overall goal of this procedure is to create localized DNA double-stranded breaks using a broadly available 405 nanometer laser scanning confocal microscope, and provide automated procedures to quantify the dynamics of DNA repair factors at the
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Studying DNA damage repair kinetics requires a system to induce lesions at defined sub-nuclear regions. We describe a method to create localized double-stranded breaks using a laser-scanning confocal microscope equipped with a 405 nm laser and provide automated procedures to quantify the dynamics of repair factors at these lesions.
