Examination of the Titer and the Screening Plates to Determine the Putative cll Mutants, Verification of Mutant Plaques, PCR Amplification, and DNA Sequencing
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Results: Use of λ Select cll Assay to Detect Mutations in the cll Transgenes from the Genomic DNA
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Conclusion
副本
The overall goal of this protocol is to visualize step-wise procedures involved in the Lambda Select cII mutation assay in cultured cells of transgenic rodents or the corresponding animals treated with chemical and physical agents of interest. Thi
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We describe a detailed protocol for the Lambda Select cII mutation assay in cultured cells of transgenic rodents or the corresponding animals treated with a chemical/physical agent of interest. This approach has been widely used for mutagenicity testing of carcinogens in mammalian cells.
