Design and Cloning of gRNAs into the CRISPR Vector
5:51
Intraperitoneal Injection of rAAVrh.74-CRISP into Neonatal mdx Mice
6:38
Analysis of Dystrophin Expression by Immunofluorescence Staining
7:31
Results: rAAV-Mediated Gene Editing Restores Dystrophin Expression in Dystrophic Mice
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Conclusion
副本
The overall goal of this in vivo cardiac gene editing protocol is to restore dystrophin expression in the heart of dystrophic mice using recombinant adeno-associated virus rh. 74 with CRISPR SaCas9 and guide RNA vectors. This method can help answe
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Here we provide a detailed protocol to carry out in vivo cardiac gene editing in mice using recombinant Adeno-Associated Virus(rAAV)-mediated delivery of CRISPR. This protocol offers a promising therapeutic strategy to treat dystrophic cardiomyopathy in Duchenne muscular dystrophy and can be used to generate cardiac-specific knockout in postnatal mice.