This method can help answer key questions in the zebrafish heart regeneration field such as the molecular mechanisms regulating heart regeneration. The main advantage of this technique is that this method is very efficient to knock down target gen
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It remains a major challenge to develop conditional gene-knockout or effective gene-knockdown in adult zebrafish organs. Here we report a protocol for performing nanoparticle-mediated siRNA gene-silencing in adult zebrafish heart, thus providing a new loss-of-function method for studying adult organs in zebrafish and other model organisms.
