Collection and Extraction of Sea Lamprey Conditioned Water
1:14
Isolation of Fraction Pools with Chromatography
2:51
Electro-olfactogram Recordings to Identify Odorous Fractions/Compounds
5:43
Two-choice Maze Behavioral Bioassay to Identify Behaviorally Active Fractions/Compounds
6:31
Chromatographic Isolation of Pure Compounds from Active Fractions
6:58
Structure Elucidation of a Pure Compound with Mass Spectrometry and Nuclear Magnetic Resonance
7:58
Results: Behavioral Responses of Ovulated Female Sea Lampreys to Odorants
8:36
Conclusion
副本
This method can help answer key questions in the chemical ecology field, such as the identification of pheromones. The main advantage of this technique is that it links novel compounds to pheromone function. To begin this procedure, place 15-30 se
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Here, we present a protocol to isolate and characterize the structure, olfactory potency, and behavioral response of putative pheromone compounds of sea lampreys.