These methods can help answer key questions about the role of the pseudokinase mixed-lineage kinase-domain like or MLKL in necroptosis such as membrane translocation and permeabilization in lipid binding. The main advantage of these techniques is
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We report methods for characterization of MLKL-mediated plasma membrane rupture in necroptosis including conventional and confocal live-cell microscopy imaging, scanning electron microscopy, and NMR-based lipid binding.
