3D Single Photon Fluorescence Implanted Thymus Confocal Imaging
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Results: Representative In Vivo Thymus Implant Imaging
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Conclusion
副本
This method can help answer key questions in immunology field about the mechanisms of autoimmunity, immunodeficiency, central tolerance, and thymus involution. The main advantage of this technique is that it permits in vivo longitudinal recording
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The goal of the protocol is to show longitudinal intravital real-time tracking of thymocytes by laser scanning microscopy in thymic implants in the anterior chamber of the mouse eye. The transparency of the cornea and vascularization of the graft allows for continuously recording progenitor cell recruitment and mature T-cell egress.