Tracking and Colocalization Analyses and Video File Preparation
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Results: Representative MB Visualization and Tracking
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Conclusion
副本
This method can help answer key questions in the RNA biology field about the mechanism and function of polarized RNA localization. The main advantage of this technique is that it allows for the real time visualization of endogenous RNA trafficking
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Here, we present a protocol for the visualization, detection, analysis and tracking of endogenous mRNA trafficking in live Drosophila melanogaster egg chamber using molecular beacons, spinning disc confocal microscopy, and open-source analysis software.
