Protein Purification by Nickel Affinity Chromatography
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Protein Activity Assay by Thin Layer Chromatography
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Results: Thin Layer Chromatography Reveals that C. difficile RSH is an Active ppGpp Synthetase
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Conclusion
副本
This method can help answer key questions about phosphotransfer enzymes including enzyme substrate specificity and reaction kinetics. The main advantage of this technique is that it allows for rapid collection of multiple data sets that are very c
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Here, we describe a method for purifying histidine-tagged pyrophosphokinase enzymes and utilizing thin layer chromatography of radiolabelled substrates and products to assay for the enzymatic activity in vitro. The enzyme activity assay is broadly applicable to any kinase, nucleotide cyclase, or phosphor-transfer reaction whose mechanism includes nucleotide triphosphate hydrolysis.