Viral Production of sgRNA Constructs and Transduction in AML-Cas9 Cells
7:10
Competitive Growth Assay
9:21
Results: The Role of Candidate Genes in AML Cell Proliferation
10:30
Conclusion
副本
This video will demonstrate a simple method for investigating multiple candidate genes in AML in parallel, using the Crisper-Cas9 system combined with the flow cytometry based competitive growth assay. The main advantage of this technique is that
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This manuscript describes a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) CRISPR-Cas9-based method for simple and expeditious investigation of the role of multiple candidate genes in Acute Myeloid Leukemia (AML) cell proliferation in parallel. This technique is scalable and can be applied in other cancer cell lines as well.
