Embedment of Cells in Collagen and Basement Membrane Matrix
7:12
Results: Primary Acinar Cells Plated within Collagen or Basement Membrane Matrix after Adenoviral Infection and Stimulation with TGF–α
7:56
Conclusion
副本
Our protocol offers direct visualization of Acinar-to-Ductal Metaplasia and allows the study of the contribution of ectopically administered factors or protein modulation to this process. The main advantage of this technique is that Acinar-to-Duct
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Primary acinar cell isolation, protein expression or activity modulation, culture, and down-stream applications are abundantly useful in the ex vivo study of acinar-to-ductal metaplasia (ADM), an early event in the development of pancreatic cancer.
