Non-malignant B and T Lymphocyte Isolation from Transgenic lck:eGFP Fish
2:11
Malignant Lymphocyte Isolation from Double-transgenic rag2:hMYC;lck:eGFP Zebrafish
4:55
Normal or Malignant B Lymphocyte Flow Cytometric Analysis
5:50
Results: Representative Malignant and Non-malignant B Cell GFP Expression in Ick:eGFP Zebrafish
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Conclusion
副本
We recently found that many B cells in LCK GFP zebrafish express low levels of GFP. This makes this line a useful tool for studying B cells as well as T cells. The main advantage of this technique is that now we can study B cells and T cells from
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Transgenic lck:eGFP zebrafish express GFP highly in T lymphocytes, and have been used to study T cell development and acute lymphoblastic leukemia. This line can be used to study B cells, which express lck at lower levels. This protocol describes purification of malignant and non-malignant B cells from lck:eGFP zebrafish.
