This protocol can be used to induce pulmonary hypertension and to perform functional measurements of the right ventricle. The Sugen Hypoxia Model is easy to implement and can recapitulate some of the hallmarks of pulmonary hypertension within two
Sign in or start your free trial to access this content
This protocol describes the induction of pulmonary hypertension (PH) in mice based on the exposure to hypoxia and the injection of a VEGF receptor antagonist. The animals develop PH and right ventricular (RV) hypertrophy 3 weeks after the initiation of the protocol. The functional and morphometrical characterization of the model is also presented.
