Pharmacological Inactive X Chromosome (Xi) Reactivation
2:50
Brain Harvest
4:45
Cryo-sectioning
5:35
Xi Reactivation Determination
6:34
Results: Representative Xi-Mecp2 Validation and Pharmacological Reactivation
7:52
Conclusion
副本
This protocol can be used to generate a non-random mouse model for testing and quantifying the reactivation of the inactive X-linked Mecp2 chromosome in the brain of a living mouse. This model can be easily modified to study Xi reactivation in oth
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Here, we describe a protocol to generate a viable female murine model with non-random X chromosome inactivation, i.e., the maternally-inherited X chromosome is inactive in 100% of the cells. We also describe a protocol to test feasibility, tolerability, and safety of pharmacological reactivation of the inactive X chromosome in vivo.
