The widely-used probe CFDA, or short for carboxyfluorescein diacetate, is non-fluorescent. The two H3 groups and the 3'6'position on this compound render it membrane-permeant. Where once inside cells, internal cellular acetates will remove the ace
Sign in or start your free trial to access this content
The goal of this protocol is to show how to load the CFDA into different sites of the bottom parts of Arabidopsis. We then present the resulting distribution pattern of CF in the shoots.
